Postdoctoral researcher in targeted mass spectrometry imaging and multi-modal spatial biology

Do you want to contribute to top quality medical research?

We have an opening for an individual wishing to join us in further developing our spatial biology workflows. The successful candidate will be expected to advance our recently established DESI-MRM mass spectrometry imaging method (see PMID:39460704). Applicants will also be involved in development of our multi-modal imaging workflow coupling DESI-MRM with spatial transcriptomics to map spatial dysregulation in metabolism. Spatial analyses will be performed in multiple model systems including ex vivo human lung tissue and in vivo animal models using both rodent as well as zebra fish-based models. These efforts will include imaging metabolic responses to specific challenges including pro-inflammatory exposures (e.g., T1 and T2 cytokines) as well as environmental chemicals of concern (e.g., PFAS, microplastics) followed by spatial transcriptomics to identify the immune cell niches associated with the formation of specific metabolites. Results will be integrated using in-house software developed in collaboration with the Spatial Biology platforms at the Science for Life Laboratory.

Position Responsibilities

Required

• Perform targeted LC-MS/MS and DESI-MRM acquisition of biological samples (including tissue extraction and cryosectioning)
• Process and analyze these data modalities (using open-source R and python packages)
• Design experiments to probe unanswered spatial questions in metabolic dysregulation

Optional

• Further develop our mass spectrometry imaging method to increase sensitivity, molecular coverage, spatial resolution and molecular specificity
• Build and implement open-source tools to develop our multi-modal efforts to spatially correlate cell, transcript and metabolite distributions
• Perform spatial transcriptomics using MERFISH (including probe selection)
• Tissue dissection and ex vivo stimulations in human lung.
• Perform zebra fish culturing and conduct targeted exposures

Your profile

We are looking for highly motivated candidates with a Ph.D. in a relevant field. Given the scope of the research, a range of different backgrounds could be appropriate, including bioanalytical chemistry, mass spectrometry, spatial biology, or bioinformatics. The successful candidate will be expected to use and further develop mass spectrometry-based methods for imaging small molecules in a range of tissues. Prior experience working with mass spectrometry imaging or spatial biology is considered a merit. Excellent communication skills and an ability to interact socially and scientifically with other members of the laboratory and with international collaborators are essential.

To be eligible for employment as a postdoctoral researcher, a PhD or a foreign degree deemed to be equivalent to a Swedish PhD is required. This eligibility requirement must be fulfilled at the latest at the time of the employment decision. Completion of your doctoral degree within the last three years is considered an advantage. If there are special reasons, your degree may have been completed earlier.

What do we offer?

We offer a creative and inspiring environment full of expertise and curiosity, which includes excellent opportunities for training in spatial biology. The laboratory offers state-of-the-art equipment, including dedicated multiple mass spectrometers (for LC-MS/MS and MSI) in a new custom designed state-of-the art analytical facility with strong collaborations with industry (enabling access to new instrumentations and training). The research environment includes a vibrant spatial biology community at the Science For Life Laboratory (SciLifeLab; https://tinyurl.com/3uvd4smu) with direct access to the latest spatial technologies, including laser microdissection, microscope slide scanning, MERFISH, VISIUM, and SCRINSHOT, as well as experienced personnel to train and assist integrating these technologies into our multi-modal workflows. Local resources include the BioImage Informatics Facility (BIIF; https://tinyurl.com/5n7swkdh), Biomedicum Imaging Center (BIC; https://tinyurl.com/3e72f275) and the Histology Core Facility (HistoCore; https://tinyurl.com/2ftj8vxb) as well as the Zebra fish core facility (https://tinyurl.com/yvmcfe3t), which is the largest in Scandinavia.

Karolinska institutet

The Karolinska Institute is one of the world´s leading medical universities. Its mission is to contribute to the improvement of human health through research and education. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is a state university, which entitles to several benefits such as extended holiday and generous occupational pension. Employees also have access to our modern gym for free and receive reimbursements for medical care. The successful applicant will belong to the Unit of Integrative Metabolomics in the Institute for Environmental Medicine. This full-time position is temporary (two years).

Location: Solna

http://www.metabolomics.se/

https://ki.se/en/imm/unit-of-integrative-metabolomics

 Choose to work at KI – Ten reasons why

Application

An employment application must contain the following documents in English or Swedish:

• PhD certificate
• A complete resumé, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
• A complete list of publications
• A summary of current work (no more than one page)

The application is to be submitted through the Varbi recruitment system.

Want to make a difference? Join us and contribute to better health for all!