Do you want to contribute to top quality medical research?

The position is within the regional and the national core facilities led by Professor Janne Lehtiö, located at the Science for Life Laboratory in Solna. The regional core facility is tasked with aiding users of Swedish universities and industry with mass spectrometry (MS) based proteomics projects. The national facility (SciLifeLab/BioMS) offers infrastructure for biological mass spectrometry (www.bioms.se). Combined, the regional and national facilities offer a unique research infrastructure and expertise with focus on MS-based proteomics and proteogenomics. The core facility works in close collaboration with Prof. Janne Lehtiö’s research group, where MS-based proteomics methods are constantly developed and applied to studies aiming at improving individualized cancer therapy. This collaboration is a prerequisite for the core facility’s ability to offer services at the forefront of MS based proteomics.

We are now looking for a research engineer with several years of research experience or equivalent to join the team working in the Mass Spectrometry core facility.

Your mission

The responsibilities accompanying this position include sample preparation, bio analytical work, analysis and reporting of protein/peptide expression, method development, use/optimization of the MS instruments, data analysis and data interpretation. Experiences in method development, data analysis and reporting are meriting.

The role also includes reporting analysis results to facility users, taking part of undergraduate/postgraduate teaching/training and representing the facilities at national and international meetings and conferences.

The research engineer will be part of a research infrastructure, working closely with project scientists, postdoctoral fellows, PhD-students, undergraduate students, and external users/customers within academia and industry. Important aspects of the job include:

  • Preparation of clinical and in-vitro samples for proteomics
  • Experimental analysis of samples by mass spectrometry
  • Compiling and analyzing proteomics data
  • Continuous contact with customers by email and phone

The work involves a combination of flexible and routine tasks and places a great emphasis on independence, initiative, precision, and planning. The position involves a variety of tasks in a stimulating research environment. Nice and helpful colleagues contribute to a good working environment within the group.

A large part of the job may be contact with customers/researchers from a wide range of fields, which is an exciting, interesting and instructive part of the work. The facility/research group is located at the Science for Life Laboratory with spacious and modern facilities.

We are seeking a highly motivated research engineer for a 1-year position to be part of our mass spectrometry core facility.

Your profile

Qualifications

  • MSc degree in analytical chemistry, biochemistry, biotechnology or in a related field
  • More than five years of research experience
  • Collaborative experience with hospitals and clinical sample handling such as urine, plasma and serum
  • Extensive hands on experience of laboratory work and ability to follow SOPs
  • Experience with techniques applied to sample preparation for proteomics, including protein extraction, clean-up (e.g. by solid phase extraction) and protein concentration measurement, as well as protein depletion (e.g. by multiple affinity removal columns, MARS) and/or equalization methodologies applied to complex proteomes
  • Experience in protein and peptide separation/fractionation using gel-based electrophoresis (1D, 2D and HiRIEF)
  • Hands-on experience with Single-Pot Solid-Phase-enhanced Sample Preparation (SP3) and in gel/in solution protein reduction/alkylation and protein digestion techniques
  • Practical experience in liquid chromatography mass spectrometry (LC-MS)
  • Good knowledge in methods used for analysis of omics data
  • Excellent communication and organizational skills
  • Good knowledge (both speaking and writing) of English

Addintional Merits

  • Competence/experience in MS-based clinical proteomics research
  • Experience in quantitative MS-based proteomics (TMT10 plex, TMT16 plex)
  • Competence in sample preparation and mass spectrometry analysis of native peptides
  • Hands-on experience with immunoassay techniques for biomarkers validation (ELISA and western blots)
  • Data management and data services
  • Strong interests of working in centralized shared research infrastructures

We place great emphasis on personal suitability. To succeed in the role, it is important that the applicant has a personal drive and flexible mind to handle the rapidly developing field of proteomics and the need of custom-based solutions in many projects. The applicant should be responsible, appreciate daily detailed work as well as have the ability to see the big picture. The applicant should be an effective communicator, have good organizational skills, and be able to prioritize at times when the workload is high. The applicant should be able to collaborate with people of different backgrounds and at different levels (both within the group and with users from various disciplines).

What do we offer?

A creative and inspiring environment full of expertise and curiosity. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is a state university, which entitles to several benefits such as extended holiday and generous occupational pension. Employees also have access to our modern gym for free and receive reimbursements for medical care.

Location: Science for Life Laboratory in Solna

https://lehtiolab.github.io

Application

Welcome to apply at the latest 4th of December.

The application is to be submitted through the Varbi recruitment system.  

Want to make a difference? Join us and contribute to better health for all

Type of employment Visstidsanställning längre än 6 månader
Contract type Full time
City Stockholm
County Stockholms län
Country Sweden
Reference number 2-5786/2019
Contact
  • Lukas Orre, +46 73 7047177, lukas.orre@ki.se
  • Helena Bäckvall, +46 8 52481209, helena.backvall@ki.se
Union representative
  • Helen Eriksson, OFR, 08-524 823 38
  • Andreas Lundqvist, SACO, 46 8 517 76884
Published 20.Nov.2019
Last application date 04.Dec.2019 11:59 PM CET

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