Karolinska Institutet, Institutionen för mikrobiologi, tumör- och cellbiologi, Pelechano

Department of Microbiology, Tumor and Cell Biology (MTC) at Karolinska Institutet conducts research and teaching in immunology, infection biology, cell biology and cancer. MTC has about 430 active employees, including 100 PhD students, 50 postdoctoral researchers and 26 professors. Key words are multidisciplinary, bridging, translational, national and international collaborations.

MTC is offering a lab manager position in the field of Genomics to work in the group of Dr. Vicent Pelechano. The group combines experimental and computational approaches to develop and apply novel genome-wide techniques to investigate eukaryotic transcription and gene Pelechano et al.Nature 2013, Chabbert et al. Mol. Syst. Biol. 2015, Pelechano et al. Cell 2015).

We are especially interested in understanding the regulatory mechanisms leading to the appearance of divergent gene expression programs in clonal cell population http://pelechanolab.com/. Different cells in a population can present varying responses to the same stimulus. In some cases, these differences are attributable to genetic mutations, such as the appearance of drug-resistant cells in bacterial and cancer populations. However, in other cases identical (clonal) cells can also display phenotypically heterogeneous responses. This non-genetic heterogeneity has a poorly understood origin but a significant impact on biology and human health. We combine the development of state-of-the art genome-wide approaches with the molecular dissection of fundamental biological problems.

Our lab is located in the newly established Science for Life Laboratory (SciLifeLab, http://www.scilifelab.se/) in the Karolinska Institute campus. SciLifeLab is equiped with state-of-the-art instrumentation and core facilities, and is home to the second largest sequencing cluster in Europe. SciLifeLab Stockholm is a joint effort between Karolinska Institute, the Swedish Royal Institute of Technology (KTH) and Stockholm University, and hosts research groups with diverse backgrounds and expertise, including basic biology, drug discovery, genomics, bioengineering, and translational medicine.

The available lab manager position concerns developing and applying novel genomic techniques to study how molecular heterogeneity (chromatin, RNA and protein expression) impacts cell-to-cell phenotypic heterogeneity in budding yeast and cancer cells. The candidate will train and assist lab members with molecular biology work such as cell culture, NGS library preparation, flow-cytometry and RNA/protein expression analysis. The candidate will collaborate with team members with inventory, orders and be responsible for the fair distribution of duties across team members. A successful candidate is expected to assist the group leader in issues such as: budget management, written reporting of laboratory activities and applications and long term planning of laboratory development. In addition to collaborate with other team members, the candidate will perform original research in genomics and lead his/her own projects regarding the development and improvement of novel genome-wide technologies. The candidate is expected to be self-driven and contribute his/her own ideas to the common goal of understanding how epigenetics, RNA biology and post-transcriptional regulation contributes to the appearance of differences across clonal populations of cells. . The ideal candidate is expected to participate in the general duties of the team and to effectively communicate with scientists of very diverse backgrounds in a highly interdisciplinary and international environment.

Entry requirements
The successful applicant should be a positive, flexible person with good communication skills and abilities to collaborate and work in a highly dynamic international research team. The candidate needs to be able to work both in a team as well as independently. Applicant must have experience of working as a lab manager or technician in academic/industry environments. Applicant’s work should be thorough and diligent, and must be good at documenting his/her own work. Applicants to this position should have a bachelor or a master’s degree in a relevant area; a PhD degree is meriting but not required.

The successful applicant should have excellent skills in experimental techniques such as NGS library preparation, flow-cytometry and mammalian cell culture. The candidate should also have good knowledge and experience of techniques within cell and molecular biology: molecular cloning, qPCR, Western blot... A strong interest in interdisciplinary technology development, and novel and creative thinking abilities are essential. Preferred experience also includes familiarity with RNA-Seq, ChIP-Seq, CRISPR and Illumina sequencing. Prior exposure to NGS data analysis, UNIX or R will be meriting. Candidate should have good skills in the English language in both writing and speaking. Documented basic Swedish skills are meriting. Personal skills will be highly valued.

Candidates interested in applying are encouraged to contact vicente.pelechano.garcia@ki.se for further information.

Application process
An employment application must contain the following documents in English:

  1. Cover letter motivating the application
  2. A complete curriculum vitae, previous academic or industrial positions, academic title, current position, academic distinctions, and committee work
  3. A complete list of publications
  4. A summary of current work (no more than one page)
  5. Verifications for crediting of illness, military service, work for labour unions or student organisations, parental leave or similar circumstances

The application is to be submitted via the MyNetwork system. Deadline for the submission is April 2nd, 2018 at midnight.

Type of employment Temporary position
Contract type Full time
First day of employment According to agreement
Salary Monthly salary
City Solna
County Stockholms län
Country Sweden
Reference number 2-523/2018
  • Vicente Pelechano, Assistant professor, vicente.pelechano.garcia@ki.se
Union representative
  • Daisy Hjelmqvist SACO, daisy.hjelmqvist@ki.se
  • Henry Wölling, SEKO, henry.wolling@ki.se
  • Niklas Andersson, OFR, niklas.andersson@ki.se
Published 25.Jan.2018
Last application date 02.Apr.2018 11:59 PM CEST

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