Postdoctoral research fellow in Translational Lung Cancer Research

Do you want to contribute to top quality medical research?

The Department of Oncology-Pathology at Karolinska Institutet conducts basic, translational and clinical research and educational activities related to cancer. The position is placed in the research laboratory of Professor Rolf Lewensohn. The group of Professor Rolf Lewensohn focuses on the development of novel treatments for lung cancer. The overall aim is to develop new oncological treatments through the approach of personalized cancer medicine.

Your mission

The candidate is expected to play a major role in molecular analysis, scientific hypothesis generation, presentation of results and manuscript preparation. The candidate will work on several projects regarding coding and non-coding RNAs, including microRNAs, and their role in acquired resistance to tyrosine kinase inhibitors. The duties involve routine cell biology work and establishment of molecular methods, as well as computational and biostatistical analyses of expression array data. The candidate will be working closely together with a senior wet lab molecular biologist, to ensure high quality output from all steps.

The position covers the following main tasks:

Taking responsibility for cell culture duties to generate and maintain non-small cell lung cancer cell lines resistant to various tyrosine kinase inhibitors
Taking responsibility for performing reproducible and robust wet lab experiments, including RT-qPCR, Western blotting, viability assays and 3’UTR reporter assays
Performing bioinformatics analyses of RNA profiles from large expression array data
Handling and extracting material from clinical lung cancer specimens
Being involved in the design and planning of wet lab experiments

Your profile

Qualified to be employed as a postdoctor is one who has obtained a doctorate or has equivalent scientific competence. Applicants who have not completed a doctorate at the end of the application period may also apply, provided that all requirements for a completed degree are met before the (intended) date of employment. This must be substantiated by the applicant's main supervisor, director or equivalent.

We are looking for an experienced postdoc-level candidate that completed his/her PhD within the last few years. The candidate should be a careful and detail-oriented person who likes to take responsibility. The candidate should be capable of close internal cooperation, as well as interacting with external collaborators that are experts in a wide variety of fields connected to our research. For this, excellent communication skills are required. The candidate should have a strong desire to deliver high quality work, on time, and have experience in working independently.

In addition, applicants must have:

Experience in working with bioinformatics tools for large expression-based array analysis, including NanoString and RT-qPCR arrays
Extensive experience with various cell culture techniques, including troubleshooting
Experience with RNA extraction, RT-qPCR, protein extraction, Western blotting, and viability assays, including troubleshooting
Experience with microRNA-based methodologies including 3’UTR assays and site-directed mutagenesis, including troubleshooting
Excellent oral and written communication skills
Fluency in English

Furthermore, it is beneficial if applicants have:

Experience in other molecular biology methods, not mentioned above
Experience in handling laboratory mice models
Experience with subcutaneous injections in laboratory mice models
Experience with intraperitoneal injections in laboratory mice models
Experience with tail-vein injections in laboratory mice models
Experience with gavage-delivery in laboratory mice models
A medical or biomedical background

What do we offer?

A creative and inspiring environment full of expertise and curiosity. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is a state university, which entitles to several benefits such as extended holiday and generous occupational pension. Employees also have access to our modern gym for free and receive reimbursements for medical care.

This employment is a temporary position for one year with the possibility of one year extension.

Location: Solna

Your career continues here

Application

An employment application must contain the following documents in English or Swedish:

A complete resumé, including date of the thesis defence, title of the thesis, previous academic positions, academic title, current position, academic distinctions, and committee work
A complete list of publications
A summary of current work (no more than one page)

Welcome to apply at the latest 2020-04-30.

The application is to be submitted through the Varbi recruitment system.

Want to make a difference? Join us and contribute to better health for all