Substitute in Pancreatic Organoid and Transcriptomics Biology

Do you want to contribute to top quality medical research?

The Andersson-Rolf lab is seeking a motivated and enthusiastic substitute with experience in spatial transcriptomics.

The newly established Andersson-Rolf lab investigates the role of cell identity in bridging developmental mechanisms with those underlying adult pancreatic regeneration and pathology. By integrating human organoid technology, (spatial)omics, genome- and bioengineering we seek to leverage insights from developmental biology to decipher the mechanisms of adult pancreatic regeneration and disease.

Your mission

We are looking for a candidate to support a project that aims to characterize pancreatic organoids and map their cellular composition using single-cell transcriptomics (scRNA-seq) and high-resolution spatial transcriptomics.

Your tasks will include:

• Establishing and maintaining human pancreatic organoid cultures
• Preparing organoid and tissue samples for scRNA-seq and spatial transcriptomics
• Developing computational pipelines for cell-type identification and spatial mapping
• Conducting microscopy and immunohistochemistry
• Performing QC, data visualization, and advanced data analysis using Python and R

Your profile

Required skills:

• Master’s degree in biotechnology, biomedicine, computational biology, or a related field
• Hands-on experience in immunofluorescence and analysis of single-cell RNAseq and spatial transcriptomics data using Python and/or R.

Desired skills:

• Experience in pancreatic or other epithelial organoid systems, microscopy, sequencing-based spatial transcriptomics, or NGS library preparation

What do we offer?

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Solna

For more information please visit:

• Amanda Andersson Rolf's Group | Karolinska Institutet
• Research Division of Medical systems bioengineering | Karolinska Institutet
• Department of Medical Biochemistry and Biophysics | Karolinska Institutet

Application

An employment application must contain the following documents in English:

• A complete curriculum vitae, including previous academic positions, academic title, current position, and academic distinctions
• A complete list of publications, if relevant
• A summary of current work (no more than one page)
• A short motivation letter (in English and no more than one page)

Welcome to apply at the latest 4th January, 2026.

The application is to be submitted through the Varbi recruitment system (i.e., not by email).

Want to make a difference? Join us and contribute to better health for all