Laboratory technician to Karolinska Institutet

Do you want to contribute to improving human health?

Karolinska Institutet, Department of Oncology-Pathology, The Thomas Helleday Laboratory

The laboratory of Professor Thomas Helleday provides an excellent translational and scientific environment. The multidisciplinary research group is located at Science for Life Laboratory and consists of Ph.D. students, postdocs, and senior scientists within biology, biochemistry, medicinal chemistry, and pharmacology, many with experience from the pharmaceutical industry.

Your mission

We are looking for a highly motivated and experienced laboratory technician for the Helleday group. Your main task will be to plan and perform in vitro cell culture experiments in the laboratory. Your work will be highly varied and will include several molecular biology techniques including cell culturing, PCR, western blotting, fluorescence microscopy, immunohistology staining techniques as well as performing various screening assays. You will also be responsible for preparing and distributing a lab duty rotation schedule for lab active personnel.

In more detail, you will contribute to a further understanding of the role of DNA repair and metabolisms proteins in cancer and characterize small molecules inhibiting these pathways and proteins involved using both biochemical and cellular assays. To support clinical findings and development we need to further investigate biomarkers, combination treatments, and potential resistance mechanisms to obtain a deeper understanding of the mechanism of action of the compounds.

Your profile

The following qualifications are required:

• A MSc in Cell or/and Molecular Biology or Biotechnology.
• Previous documented laboratory experience.
• Previous documented experience establishing disease models within oncology
• Previous documented experience with cell work, including handling and culturing of various cells including blood and solid cancer cells.
• Previous documented experience with standard cellular techniques, such as cellular viability/survival and clonogenic assays and viral transfection techniques.
• Previous documented experience with molecular biology techniques, eg qPCR, cloning, protein purification, cellular target engagement analysis using CETSA, and Western blotting is a requirement.
• Previous documented experience with immunohistology methods and confocal microscopy is a requirement.
• Previous documented experiences of techniques such as DARTS and cell synergy experiments are merit.
• Previous documented experience of running enzymatic activity assays is a merit.
• You need to be able to work in an organized and timely manner and to have experience of working in multidisciplinary teams.
• You need to have excellent knowledge of English, both spoken and written.
• You need to have good knowledge of Microsoft Office programs, such as Word, Excel, Powerpoint, and GraphPad Prism.
• We will place great emphasis on personal qualities

What do we offer?

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: SciLifeLab, Solna

Choose to work at KI – Ten reasons why
Department of Oncology-pathology

Application

Welcome to apply at the latest 23^th January. 

The application is to be submitted through the Varbi recruitment system.In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form. 

Permanent position is initiated by a six months trial period.

Want to make a difference? Join us and contribute to better health for all