Research Engineer in high throughput yeast Genomics - Hiring in process/Finished, not possible to apply

Do you want to contribute to top quality medical research?

The Department of Microbiology, Tumor and Cell Biology (MTC) at Karolinska Institutet conducts research and teaching within immunology, infection biology, cell biology and cancer. MTC has about 40 research groups and our key words are multidisciplinary, bridging, national and international collaborations.Our laboratory is located at the Science for Life Laboratory in the Karolinska Institute campus in Solna. SciLifeLab is equiped with state-of-the-art instrumentation and core facilities, and is home to one of the largest sequencing cluster in Europe.

Our group combines experimental and computational work, and aims to develop and apply novel genome-wide techniques to address fundamental biological questions with medical implications. We are especially interested in how subtle variations in gene expression can lead to differential cellular phenotypes and are passionate about RNA biology. Read more about our work here. You will have the opportunity to work in a highly interdisciplinary and international environment and combine the development of state-of-the art genome-wide approaches with the molecular dissection of fundamental biological problems.

Are you interested in unravelling the eukaryotic post-transcriptional regulatory code? Would you like to combine the use of cutting-edge genome-wide technologies with synthetic biology and machine learning to study RNA life? Come and join us at SciLifeLab.

Your mission

Description of the subject area
Posttranscriptional gene expression regulation describes the various mechanisms that influence gene expression after transcription but before translation into protein. These processes include RNA splicing, editing, transport, stability, and translation efficiency. Posttranscriptional regulation allows cells to adjust mRNA fate and protein production without affecting transcription. The budding yeast Saccharomyces cerevisiae it is an ideal model to studying posttranscriptional gene regulation at genome-wide scale. Its compact genome and the accessibility of genetic and synthetic biology tools have established S. cerevisiae as a primary organism for investigating these regulatory layers. Our team aims to leverage recent advances in high-throughput genomics and artificial intelligence to enhance our understanding of how posttranscriptional regulation influences gene expression patterns and cellular characteristics.

Your mission
The available research engineer position concerns developing and applying novel genomic approaches to study gene expression and mRNA life across Ascomycota. The candidate will work in the context of a recently awarded ERC synergy project (EPIC - Unravelling the eukaryotic post-transcriptional regulatory code) in collaboration with the labs of Prof. Kevin Verstrepen (Leuven) and Prof. Julien Gagneur (Munich). The candidate will focus on stablishing novel experimental pipelines to enable the high-throughput generation of high-quality genomic data. This will include development and automatization of novel NGS approaches.

The candidate will perform original research, contribute to the training of junior colleagues, and acquire the required experimental and computational skills necessary for the completion of the project. The candidate will coordinate sample and data exchange between Sweden, Germany and Belgium in the context of an awarded ERC Synergy grant. The candidate is expected to communicate the scientific results contributing to the writing scientific papers and by attending scientific meetings. Further responsibilities include supporting laboratory management and coordination, and assisting the group leader with administrative duties. The ideal candidate will participate in the general duties of the team and effectively communicate with scientists of very diverse backgrounds in a highly interdisciplinary and international environment. Working language is English.

Your profile

Applicants to this position should have a bachelor’s degree in Biotechnology, Molecular Biology or similar field.

In addition to the above requirements for the position:

• A strong interest in interdisciplinary technology development, and novel and creative thinking abilities.
• Extensive experience in NGS method development.
• Experience in methods such WGS, RNA-Seq, polyA mapping, TSS mapping, 5PSeq, CUT&RUN and Tn5-based NGS library construction approaches.
• Hands-on experience in liquid handling robotics.
• Experience in yeast genetics, with a focus on S. cerevisiae.
• Experience in DNA/RNA extraction.
• Experience with Illumina sequencers.
• Good interpersonal skills and ability to work in teams.

Meritorious for the position:

• Experience in transcription and post-transcriptional mRNA regulation.
• Experience in alternative polyadenylation.
• Experience in high-throughput yeast phenotyping and QTL mapping.
• Experience in Nanopore sequencing
• Experience in project management and data reporting.
• Experience coordinating small teams in the academic and/or industrial sector

In this recruitment, special weight will be attached to scientific skill of relevance to the project and to previous research experience.

What do we offer?

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Solna

Application

Welcome to apply at the latest 2025-11-05

The application is to be submitted through the Varbi recruitment system.In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form. 

Permanent position is initiated by a six months trial period.

Want to make a difference? Join us and contribute to better health for all