Research Assistant in phage biology and protein engineering

Do you want to contribute to top quality medical research?

The Department of Microbiology, Tumor and Cell Biology (MTC) at Karolinska Institutet conducts research and teaching within immunology, infection biology, cell biology and cancer. MTC have about 40 research groups and our key words are multidisciplinary, bridging, national and international collaborations.

Your mission

The research group of Dr Andrea Fossati seeks a research assistant in the field of phage gene products and new antimicrobials. The Fossati lab (https://fossatilab.app) investigates bacterial immune systems composition and mechanism by employing a combination of bulk NGS technologies and high-throughput interaction proteomics approaches (Fossati 2023, Nat Com) as well as developing computational solutions to analyze next-generation interaction proteomics datasets using machine learning (Fossati 2021, Nat Methods) and deep learning (Frommelt and Fossati Nat Methods 2024). The team employs an integrative and collaborative methodology, maintaining close partnerships with computational and experimental units at KI, Science for Life Laboratory (SciLifeLab), as well as local and international research collectives. Regular on-site courses are offered, complemented by opportunities for further professional development via summer schools and ongoing workshops.

Your profile

The available research assistant position concerns developing and testing new phage-inspired antimicrobials on multi-drug resistant pathogens of clinical concern (E. coli, K. pneumoniae, P. aeruginosa, A. baumannii, M. tuberculosis, M. abscessus). Overall, we aim to new develop phage therapeutics which are well understood and ready to be tested in pre-clinical models. As a research assistant, you will oversee data generation, conduct analyses, and provide insightful interpretations. Collaboration with fellow team members, other groups at KI/SciLifeLab will be a key aspect of your role. Adaptability to a diverse, interdisciplinary, and global research environment, along with a commitment to ongoing professional growth, is essential.

Requirements for the position (knowledge, skills, abilities): 

• Prior expertise with phage lysins
• Expertise in high throughput screenings
• Working knowledge with Python/R programming
• Basic molecular cloning (primers design, PCR, restriction, ligation, Golden Gate assembly, competent cells preparation, bacterial transformation, plasmid purification, gel electrophoresis,etc)
• Basic biochemistry (protein expression, purification, western blots, immunofluorescence)
• Basic microbiology techniques

• Prior experience with Mycobacteria
• Experience with bacterial genetics
• Knowledge of virology/phage biology techniques (plaque assay, phage isolation, etc)
• Hands-on experience in proteomics sample preparation or data analysis is a plus

What do we offer?

A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.

Location: Solna

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Application

Welcome to apply at the latest 2024-11-30.

The application is to be submitted through the Varbi recruitment system.In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form. 

Want to make a difference? Join us and contribute to better health for all