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Karolinska Institutet, Department of Cell and Molecular Biology (CMB)
CMB is a department with a strong focus on basic science. The research groups are organized in three thematic programs: Developmental and Stem Cell Biology, Cell Biology and Infection and Cancer. CMB conducts research and education in cell biology, molecular biology, developmental biology, stem cell biology, cancer and infection biology. A research assistant position is available in the research group of Dr. Elif Eroglu. The team consists of scientists who are focused on studying cardiogenesis in multiple model organisms and its implications for regenerative medicine. The research goal is to find novel pathways and principles for regenerative medicine in general, and to repair damaged cardiac muscle cells in particular. The position is time limited.
Duties
Your main priority will be to study epicardial-myocardial cell interactions based on cardiac organoid cultures both in the context of cardiac development and regeneration. Duties in the laboratory will include cell culture, human stem cell differentiation, cell transfection and evaluation of in vitro proliferation, migration and viability assays. Additionally, you will be required to assist in the laboratory with basic molecular biology techniques, tissue processing and cryosectioning, immunofluorescence staining, confocal microscopy and image analysis. Depending on the project progress you will be expected to learn and apply new techniques. The working language in the lab is English.
Entry requirements
A suitable background is a Master’s degree within an area which the employer deems to be relevant. You must have documented experience studying cell-cell junctions in vitro using human cell culture, including the cultivation of epithelial and fibroblast cell lines. Hands-on experience working with human stem cells and basic understanding of cell differentiation protocols is meriting. You must master basic molecular biology techniques including cloning, western blot, qPCR and immunoassays. Previous experience using fluorescent protein tags and SNAP-labelling of plasma membrane-localizing proteins is highly desired. Expertise in the design and implementation of experiments based on immunolabelling, confocal and STED microscopy is required.
Skills and personal qualities
You are thorough, diligent and used to documenting and organizing your work. You work in a clean and precise way. We expect you to be a positive, flexible person with good communication skills who appreciates being in a highly dynamic international research team. Due to the progressive nature of a scientific project we expect you to be comfortable with learning new methods. You can work independently after training but are still a team player and very service minded. Strong organizational skills as well as the ability to adapt quickly to changing priorities, assignments, and roles will be critical.
Furthermore, you have excellent communication skills in English in both writing and speaking. Personal skills will be highly valued.
A creative and inspiring environment with wide-ranging expertise and interests. Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote a better health for all. At Karolinska Institutet, we conduct successful medical research and hold the largest range of medical education in Sweden. Karolinska Institutet is also a state university, which entitles you to several good benefits through our collective agreement. And you get to practice freely in our modern wellness facilities, where trained staff are on site.
Location: Solna
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Welcome to apply!
The application is to be submitted through the Varbi recruitment system.In this recruitment, you will apply with your CV without a personal letter. Instead, you will answer some questions about why you are applying for the job in the application form.
Type of employment | Special fixed-term employment |
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Contract type | Full time |
First day of employment | Upon agreement |
Salary | Monthly salary |
Number of positions | 1 |
Full-time equivalent | 100% |
City | Stockholm |
County | Stockholms län |
Country | Sweden |
Reference number | STÖD 2-2453/2024 |
Contact |
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Union representative |
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Published | 11.Jun.2024 |
Last application date | 25.Jun.2024 11:59 PM CEST |