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The Department of Biosciences and Nutrition performs research and education in several areas of medical science including aging, Alzheimer, bioorganic chemistry, biomaterial, molecular endocrinology, cancer biology, functional genomics, systems biology, epigenetics, structural biochemistry, immunology, and nutrition. It offers an excellent international research and working environment, including around 250 scientists, students, administrative and technical personnel. The Department resides in Neo at Campus Flemingsberg, a biomedical research building, aimed at being a creative and open environment that enables meetings, synergies, and exploration of areas of mutual interest across disciplines.
We are looking for an ambitious research assistant with knowledge and skills in working with protein misfolding diseases using Drosophila melanogaster as a model system.
The Rising’s research group https://medarbetare.ki.se/people/annris has several exciting studies that aim to increase our understanding of protein folding, including the production and spinning of artificial spider-silk super fibers. In contrast, other studies aim to increase our knowledge of amyloid formation and how different amyloid fibrils interact. AA amyloidosis occurs in cattle, and there is a risk that when diseased animals are used for consumption AA amyloid becomes biohazardous and triggers misfolding of other amyloid-prone proteins.
You should independently perform biochemical and biophysical experiments and conduct animal experiments in a Drosophila model. The tasks include handling the fly stocks, producing standard and special diets, and crossing flies to generate lines with the desired expression pattern. You will acquire and analyze data from these experiments using e.g. MATLAB, and you should do confocal microscopy and transmission electron microscopy.
You should have advanced experience in working with Drosophila melanogaster. We use the Gal4UAS system for transgene expression and multiple driver lines to obtain tissue-specific expression patterns. Developed phenotypes should be analyzed using semi-automated optical heartbeat analysis (SOHA) to calculate systolic and diastolic interval, heart rate and heartbeat rhythmicity, and Drosophila activity monitoring system (DAMS) to study circadian rhythm. Data obtained from SOHA and DAMS will be analyzed using MATLAB. The morphological analysis will be performed on a variety of organs, including the dissected fly brain, which requires excellent fine motor skills. Protein aggregation is studied using confocal microscopy and transmission electron microscopy. Experience in amyloid protein research and fluency in English is a requirement.
Karolinska Institutet is one of the world's leading medical universities. Our vision is to pursue the development of knowledge about life and to promote better health for all. At Karolinska Institutet, we conduct innovative medical research and provide the largest range of biomedical education in Sweden. Karolinska Institutet is a state university, which entitles employees to several benefits such as extended holiday and a generous occupational pension. Employees also have free access to our modern gym and receive reimbursements for medical care.
Location: Flemingsberg
We prefer that your application is written in English, but you can also apply in Swedish. An application must contain the following documents:
The application is to be submitted through the Varbi recruitment system.
Permanent position is initiated by a six months trial period.
Type of employment | Temporary position |
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Contract type | Part-time |
Salary | Monthly salary |
Number of positions | 1 |
Full-time equivalent | 60% |
City | Huddinge |
County | Stockholms län |
Country | Sweden |
Reference number | STÖD 2-958/2021 |
Contact |
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Union representative |
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Published | 04.Mar.2021 |
Last application date | 18.Mar.2021 11:59 PM CET |