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Description of the division
iPS core is a human cell facility, (www.ipscore.se), located at Karolinska Institutet in Solna. The facility was founded in 2013 by associate professor Anna Falk. The focus of the cell facility is culturing of human pluripotent stem cells, so called human induced pluripotent cells (iPS cells) and neuronal stem cells derived thereof. The services are aiming at deriving and providing standardized cell cultures, cell culture advice and protocols for preclinical research.
Area of responsibilities/duties
Majority of the work will be administration, customer contacts, and advanced cell culturing (iPS cells and neural cells), and characterization of the generated cells. The work includes the whole reprogramming process from establishment of differentiated cells (for example fibroblast cells from skin biopsies) to reprogramming and expansion, characterisation and cryopreservation of established pluripotent stem cells cultures and other cellular models like neural stem cells. Hands on cell culture, demonstrations and teaching for researchers. Active contribute to process development and standardization of existing and new processes. Contribute to a high standard of services, documentation and SOP derivation.
Further duties are neural differentiation of pluripotent stem cells in 2D and 3D and various cell-based assays as flow cytometry, immunocytochemistry, MEA, cloning, CRISPR/Cas9 editing, protein work and qRT-PCR. Additional responsibilities are administration as ordering, handling invoices, write offers and contracts, updating website, organising courses and symposia, taking care of lab equipment, analysing large set of data. Furthermore, creating visions, strategies and drive RnD work for future services and additional platforms and cell models offered by the iPS Core in addition to formulating and writing application and reports.
Examples of specific duties:
Culture and cryopreservation of human iPS and rarely ES cells.
Derivation of human fibroblast cells from skin biopsies
Reprogramming of human fibroblast cells
Characterisation of iPS cells using immunocytochemistry, flow cytometry and quantitative RT-PCR.
Differentiation of stem cells to neural stem cells and other cell types in 2D and 3D
Preparation of DNA and RNA
Cloning and preparation of DNA
CRISPR/Cas9 genome editing
Ordering and tracking media, reagents and plastics and maintaining inventory
Updating website
Documentation, electronic lab book
Creation of SOPs
Medium preparation and factor aliquotation
Hands on cell culture training for researcher/ cell customers
Together with customers design, advice and formulate projects
Arrange with MTA-documents and cell shipments to customers
GMP cell production might be a part of the cell culture duties
Care for lab equipment
Help with organisation of courses and symposia
Basic analysis of large data sets
Cell based assays of migration, proliferation and differentiation
Entry requirements
The applicant should have a PhD in stem cell biology in addition to some years post-doctoral experience.
Documented experience of the following is required:
The following experience is preferable and meritorious:
Most of the work is advanced and labour intense cell culture with much routine and there is no opportunity to work on individual research projects.
The applicant should be fluent in English, with good communication and writing skills since the working environment is international.
Great importance will be placed on personal qualities.
An overall assessment of the applicants’ qualifications will be made.
Application process
An employment application must contain the following documents in Swedish or English:
The application is to be submitted through the Varbi recruitment system.
Type of employment | Permanent position |
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Contract type | Full time |
First day of employment | as soon as possible |
Salary | Monthly salary |
Number of positions | 1 |
Full-time equivalent | 100 % |
City | Stockholm |
County | Stockholms län |
Country | Sweden |
Reference number | 2-5114/2019 |
Contact |
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Published | 11.Oct.2019 |
Last application date | 21.Oct.2019 11:59 PM CEST |