Research assistant - iPSC disease modelling - Under tillsättning eller tillsatt, ej sökbar

Avdelning
The position is in the Rare Diseases research group. We study genetic disorders on a molecular level in order to understand disease mechanisms and apply new knowledge to genetic diagnostics. We work in a modern laboratory at the Bioclinicum building situated within the grounds of the Karolinska University Hospital, Solna. Several group members also work in the Department of Clinical Genetics at the hospital, the largest clinical genetic department in Sweden with 90 employees working with genetic diagnosis and counseling. We also work closely with the Science for Life Laboratory in Solna, and thus have access to massively parallel DNA sequencing (MPS) with all available technologies. We are currently developing in vitro modeling assays in cells to evaluate genetic mutations detected in patients. Read more:https://ki.se/en/mmk/rare-diseases

Arbetsuppgifter
We are looking for an enthusiastic and talented person with a background in molecular and developmental biology. The successful applicant will be involved in analyzing the function of new candidate genes and mutations that might be responsible for hereditary neurodevelopmental disorders in patients and families that are counseled in our clinic. The selected applicant will help to stablish robust differentiation techniques from hiPSC and neural stem cells to generate relevant cell types to model neurodevelopmental disorders. Experience in cellular models with culturing and analyzing primary cells, human pluripotent cells (hiPSCs and hESCs),  human neural stem cells as well as cerebral organoid 3D culture is therefore desired. This project provides the opportunity to participate in a translational project that will have direct clinical impact.

Kvalifikationer
A degree within the area of biomedical research or corresponding area is required.
We are seeking a new group member with a broad range of interests and expertise in the following key areas:
1) Advanced cell culture: 2D Culturing of hiPSCs and human neural stem cells
2) 3D cerebral organoids culture
3) Differentiation of iPSCs and neural stem cells into various neural subtypes and characterization of the derived cells.
4) Image acquisition and analysis: Expertise in immunoflourescent and confocal microscopy, time-lapse imaging, image processing and analysis, including statistical analysis.
5) Molecular Biology/Cell Biology: Knowledge in basic molecular techniques specifically molecular gene cloning, in vitro mRNA synthesis and gene expression assays including PCR, RT-PCR, and qRT-PCR, as well as Western Blot protein analysis.
Applicants should have prior experience/training in these model systems/areas. The applicant will be evaluated based on education and previous experience, relevance for the project, publications and track records, motivation, problem solving skills, and cooperative ability. Personal suitability is also an important criterion.

Ansökningsförfarande
Ansökan ska göras via rekryteringssystemet Varbi.